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positive displacement pipettes  (Gilson Inc)


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    Structured Review

    Gilson Inc positive displacement pipettes
    Positive Displacement Pipettes, supplied by Gilson Inc, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microman+positive+displacement+pipette/Microman+E+M100E/bio_rxiv__64898__2026__03__21__712762-170-24-26
    Average 94 stars, based on 34 article reviews
    positive displacement pipettes - by Bioz Stars, 2026-09
    94/100 stars

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    Transferring:

    Article Title: Improving the efficiency of quantitative (1)H NMR: an innovative external standard-internal reference approach.
    Article Snippet: The classical internal standard quantitative NMR (qNMR) method determines the purity of an analyte by the determination of a solution containing the analyte and a standard.. Therefore, the standard must meet the requirements of chemical compatibility and lack of resonance interference with the analyte as well as a known purity.. The identification of such a standard can be time consuming and must be repeated for each analyte.

    Article Title: Structural characterization of low level degradants in aztreonam injection and an innovative approach to aid HPLC method validation.
    Article Snippet: Three new degradants have been identified from drug product and active pharmaceutical ingredient stability samples of aztreonam, a marketed synthetic monocyclic beta-lactam antibiotic.. The degradants were detected following the implementation of a new, more selective HPLC method for the determination of impurities and degradants.. The new method was developed in response to changes in the regulatory requirement for mature products.

    Article Title: Fluorescent Labeling of Rat-tail Collagen for 3D Fluorescence Imaging
    Article Snippet: MatTek Dishes (35 mm, #1.5 coverslip, 20 mm opening: MATTEK, catalog number: P35G-1.5-20-C) ColorpHast pH-indicator-strips with pH range 6.5–10 (Merck, catalog number: 109543) 10–100 and 1,000 μl Gilson MICROMAN ® positive displacement pipette tips (Gilson, catalog numbers: FD10004, FD10006) 10 cm tissue culture dish (Thermo Fisher Scientific, catalog number: 150350) Cell lifters (Corning, catalog number: 3008) Aluminum foil Plastic wrap Scintillation vial (Sigma-Aldrich, catalog number: Z190527) 1.5 ml microfuge tubes (Thermo Fisher Scientific, catalog number: AM12400) Dubecco’s Minimal Essential Medium (DMEM) powder, Phenol red (Sigma-Aldrich, catalog number: D2429) NaOH pellets (Sigma-Aldrich, catalog number: S8045) Phosphate Buffered Saline with Calcium and Magnesium (PBS ++ ) chilled to 4 °C and at room temperature (GE Healthcare, Hyclone TM , catalog number: SH30264.02) Rat tail collagen solution (dissolved in 20 mM acetic acid, commercial brands are fine, but in-house preparations are usually cleaner and polymerize faster) at a concentration greater than 5 mg/ml (6 mg/ml used here) Sodium bicarbonate (Sigma-Aldrich, catalog number: S5761) 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Sigma-Aldrich, catalog number: H4034) Boric acid (powder 99.5%, Sigma-Aldrich, catalog number: B6768) Acetic acid (Sigma-Aldrich, catalog number: 27221-1L) (chilled to 4 °C, less than 50 ml of 16.65 M is required) Atto-488 NHS-ester 1 mg (Sigma-Aldrich, catalog number: 41698) DMSO (Sigma-Aldrich, catalog number: D2650) Sircol Collagen Assay kit (available from Accurate Chemical & Scientific, catalog number: CLRS1000) Slide-A-Lyzer Dialysis cassette (G2) with 20,000 MW cutoff (Thermo Fisher Scientific, catalog number: 87735) 1 M Tris (pH 7.4, KD Medical, catalog number: RGF-3340) NaCl (Sigma-Aldrich, catalog number: S7653) HCl (37%, Sigma-Aldrich, catalog number: H1758-500ML) 10x DMEM (see Recipes) 10x reconstitution buffer (10x RB; see Recipes) 1 N NaOH (500 μl in a microfuge tube; see Recipes) 1 N HCl (500 μl in a microfuge tube; see Recipes) 50 mM Borate buffer pH 9.0 (see Recipes) 5 mg/ml Atto-488 NHS-ester dye in DMSO (see Recipes) Sodium Chloride (NaCl), 8% solution (see Recipes) 50 mM Tris buffer (see Recipes) .. 10–100 and 1,000 μl Gilson MICROMAN ® positive displacement pipette (Gilson, catalog numbers: F148314, F148180) Lab timer 2–4 L beaker Ultra-clear 8 × 20 mm centrifuge tubes (Optional: Beckman Coulter, catalog number: 345843) Rectangular ice bucket packed with ice Water bath Large Magnetic stir bar to large beaker (Sigma-Aldrich, SP Scienceware - Bel-Art Products - H-B Instrument, catalog number: Z284491) Small Magnetic stir bar for scintillation vial (Sigma-Aldrich, SP Scienceware - Bel-Art Products - H-B Instrument, catalog number: Z126942) Stir plate Mini centrifuge ( i.e. , VWR, model: Galaxy Mini) Biological hood (any brand) Brightfield microscope with 10 and 20x phase contrast objectives Cooled centrifuge capable of 20,000 × g and 4 °C ( i.e. , TOMY, model: MX-307) Lab Rocker ( i.e. , Denville Scientific, model: 110, catalog number: S2110) Airfuge ultra centrifuge (optional: Beckman Coulter, catalog number: 340400) Laser scanning or spinning disk confocal microscope ( i.e. , Nikon Instruments, model: A1R or Yokogawa, model: CSU-X1) ..

    Article Title: Vitamin A-fortified milk increases total body vitamin A stores in Mexican preschoolers.
    Article Snippet: .. After an overnight fast, children were given an oral dose of stable isotope-labeled VA in 200 mL of corn oil using a Gilson Microman positive displacement pipette. ..

    Article Title: Fluorescent Labeling of Rat-tail Collagen for 3D Fluorescence Imaging
    Article Snippet: .. 10–100 and 1,000 μl Gilson MICROMAN® positive displacement pipette (Gilson, catalog numbers: F148314, F148180) 2. ..

    Article Title: DEHP Nanodroplets Leached from Polyvinyl Chloride IV Bags Promote Aggregation of IVIG and Activate Complement in Human Serum
    Article Snippet: .. Using a Gilson Microman 1 mL positive displacement pipette, 3 mL of DEHP was combined with 97 mL of ultrapure water in a stainless-steel cylinder. .. This suspension was mixed under high shear using a Virtishear mechanical homogenizer (The Virtis Company, Gardiner, New York) for 15 minutes at 30,000 rpm.

    Microscopy:

    Article Title: Fluorescent Labeling of Rat-tail Collagen for 3D Fluorescence Imaging
    Article Snippet: MatTek Dishes (35 mm, #1.5 coverslip, 20 mm opening: MATTEK, catalog number: P35G-1.5-20-C) ColorpHast pH-indicator-strips with pH range 6.5–10 (Merck, catalog number: 109543) 10–100 and 1,000 μl Gilson MICROMAN ® positive displacement pipette tips (Gilson, catalog numbers: FD10004, FD10006) 10 cm tissue culture dish (Thermo Fisher Scientific, catalog number: 150350) Cell lifters (Corning, catalog number: 3008) Aluminum foil Plastic wrap Scintillation vial (Sigma-Aldrich, catalog number: Z190527) 1.5 ml microfuge tubes (Thermo Fisher Scientific, catalog number: AM12400) Dubecco’s Minimal Essential Medium (DMEM) powder, Phenol red (Sigma-Aldrich, catalog number: D2429) NaOH pellets (Sigma-Aldrich, catalog number: S8045) Phosphate Buffered Saline with Calcium and Magnesium (PBS ++ ) chilled to 4 °C and at room temperature (GE Healthcare, Hyclone TM , catalog number: SH30264.02) Rat tail collagen solution (dissolved in 20 mM acetic acid, commercial brands are fine, but in-house preparations are usually cleaner and polymerize faster) at a concentration greater than 5 mg/ml (6 mg/ml used here) Sodium bicarbonate (Sigma-Aldrich, catalog number: S5761) 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Sigma-Aldrich, catalog number: H4034) Boric acid (powder 99.5%, Sigma-Aldrich, catalog number: B6768) Acetic acid (Sigma-Aldrich, catalog number: 27221-1L) (chilled to 4 °C, less than 50 ml of 16.65 M is required) Atto-488 NHS-ester 1 mg (Sigma-Aldrich, catalog number: 41698) DMSO (Sigma-Aldrich, catalog number: D2650) Sircol Collagen Assay kit (available from Accurate Chemical & Scientific, catalog number: CLRS1000) Slide-A-Lyzer Dialysis cassette (G2) with 20,000 MW cutoff (Thermo Fisher Scientific, catalog number: 87735) 1 M Tris (pH 7.4, KD Medical, catalog number: RGF-3340) NaCl (Sigma-Aldrich, catalog number: S7653) HCl (37%, Sigma-Aldrich, catalog number: H1758-500ML) 10x DMEM (see Recipes) 10x reconstitution buffer (10x RB; see Recipes) 1 N NaOH (500 μl in a microfuge tube; see Recipes) 1 N HCl (500 μl in a microfuge tube; see Recipes) 50 mM Borate buffer pH 9.0 (see Recipes) 5 mg/ml Atto-488 NHS-ester dye in DMSO (see Recipes) Sodium Chloride (NaCl), 8% solution (see Recipes) 50 mM Tris buffer (see Recipes) .. 10–100 and 1,000 μl Gilson MICROMAN ® positive displacement pipette (Gilson, catalog numbers: F148314, F148180) Lab timer 2–4 L beaker Ultra-clear 8 × 20 mm centrifuge tubes (Optional: Beckman Coulter, catalog number: 345843) Rectangular ice bucket packed with ice Water bath Large Magnetic stir bar to large beaker (Sigma-Aldrich, SP Scienceware - Bel-Art Products - H-B Instrument, catalog number: Z284491) Small Magnetic stir bar for scintillation vial (Sigma-Aldrich, SP Scienceware - Bel-Art Products - H-B Instrument, catalog number: Z126942) Stir plate Mini centrifuge ( i.e. , VWR, model: Galaxy Mini) Biological hood (any brand) Brightfield microscope with 10 and 20x phase contrast objectives Cooled centrifuge capable of 20,000 × g and 4 °C ( i.e. , TOMY, model: MX-307) Lab Rocker ( i.e. , Denville Scientific, model: 110, catalog number: S2110) Airfuge ultra centrifuge (optional: Beckman Coulter, catalog number: 340400) Laser scanning or spinning disk confocal microscope ( i.e. , Nikon Instruments, model: A1R or Yokogawa, model: CSU-X1) ..



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